![]() ![]() For each dilution step, we take 1 ml of the solution from the previous step and add it to 9 ml of diluent. ![]() In this example, we start with 10 ml of our stock solution. Here’s an example of how to create a 1:10 serial dilution: Dilution Step Use : Serial Dilution Calculator Serial Dilution Example Lastly, it’s important to note that while this guide provides a general method for performing serial dilutions, some adjustments might be necessary based on your specific experiment or the nature of the substance you’re diluting. Always use sterile equipment, work in a clean environment, and plan your dilution series carefully. Remember, while making serial dilutions may seem straightforward, it requires careful technique to avoid contamination and ensure accuracy. Some may need to be used immediately, while others might be refrigerated or frozen for later use. Upon completion of the dilution series, ensure that your samples are properly stored. You should record both the dilution factor and the absolute concentration if it’s known. ![]() Step 6: Label Each Dilutionĭon’t forget to label each test tube or well accurately and clearly. Step 5: Repeat the ProcessĬontinue this process, repeating step 4, for as many dilutions as you require, ensuring that you thoroughly mix each dilution before moving on to the next one. For example, to create a 1:100 dilution, you would take 1 mL of your 1:10 dilution and add it to 9 mL of diluent. Remember to change pipette tips between each transfer to avoid contamination. Take a new pipette and transfer an aliquot from your first dilution to a new test tube containing fresh diluent. Mix this thoroughly to ensure an even concentration. For example, if you want a 1:10 dilution, you might add 1 mL of stock solution to 9 mL of diluent. The amount you’ll transfer depends on the dilution factor you want. Using a new, sterile pipette tip, transfer a certain volume of your stock solution into a new test tube containing the diluent. It could be a simple buffer, a culture medium, or even distilled water. The choice of diluent depends on your experiment. The diluent should be prepared in a sterile environment if working with biological samples to prevent contamination. Decide the total number of dilutions you need for your analysis. A typical serial dilution might look like this: 1:10, 1:100, 1:1000, and so on, where each dilution is a tenth of the previous one. Steps for Making Serial Dilutions Step 1: Plan Your Dilutionįirst, determine the series of dilutions you require for your experiment.
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